Cell-free Hemoglobin Synthesis
نویسندگان
چکیده
A cell-free protein-synthesizing system derived from rabbit reticulocytes is described which is dependent on the addition of transfer RNA for the translation of endogenous hemoglobin messenger RNA. Product analysis indicates that the system is active in the initiation of new chains. When hemoglobin is synthesized in the presence of a limiting amount of tRNA, there is a 50% decrease in (Y chain production relative to /3 chain production. Deacylated rabbit liver tRNA can, but deacylated Escherichia coli tRNA cannot, be substituted for rabbit reticulocyte tRNA in the synthesis of hemoglobin.
منابع مشابه
Studies of the terminal stages of hemoglobin synthesis: I. Stimulation of globin chain synthesis by native hemoglobin subunits.
A homologous canine reticulocyte cell-free system was utilized to determine the effects of native hemoglobin subunits on the synthesis of the a and chains of globin. The addition of either a” or in concentrations of iO -iO M to the cell-free system caused a reproducible increase in synthesis of globin to a maximum of 1 ‘/2 tImes that of control values. Tetrameric hemoglobin and other nonglobin ...
متن کاملInhibition of hemoglobin synthesis by cyanate in vitro.
Cyanate inhibits sickling and prolongs red cell cell was not impaired, and free intracellular survival in sickle cell anemia. However, cyanate amino acid was not carbamylated. Aminoacylamarkedly inhibits hemoglobin synthesis in vitro. tion of transfer RNA was not inhibited; the Incorporation of radioactive amino acid into acylated amino acid was not carbamylated. hemoglobin by human sickle reti...
متن کاملCell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.
A cell-free protein-synthesizing system derived from rabbit reticulocytes is described which is dependent on the addition of transfer RNA for the translation of endogenous hemoglobin messenger RNA. Product analysis indicates that the system is active in the initiation of new chains. When hemoglobin is synthesized in the presence of a limiting amount of tRNA, there is a 50% decrease in (Y chain ...
متن کاملDefect in messenger RNA for human hemoglobin synthesis in beta thalassemia.
Functional messenger RNA for human hemoglobin synthesis was prepared from reticulocyte lysates of patients with homozygous beta thalassemia and sickle cell anemia. The messenger RNA stimulated the synthesis of human globin chains by a cell-free system derived from Krebs mouse ascites cells. In the presence of beta thalassemia messenger RNA, the system synthesized much less beta chain than alpha...
متن کاملGlobin messenger RNA in hemoglobin H disease.
Functional messenger RNA (mRNA) for human globin synthesis was isolated from reticulocytes of each of two patients with hemoglobin H disease. The RNA was tested for its capacity to direct globin synthesis in a messenger RNA-dependent cell-free system derived from Krebs Type II mouse ascites tumor cells. In each case, hemoglobin H disease mRNA directed the synthesis of a great excess of /3-globi...
متن کامل